Trichinellosis is a food-borne parasitic zoonosis with a yearly incidence of about 10,000 clinical cases worldwide. It is one of the most serious zoonotic diseases in Romania with more than 28,000 human cases reported over the last 25 years. Trichinella species and genotypes are present on all continents, from the tropical regions to the cold ones. Up to date, there have been identified four Trichinella species in Europe: T. spiralis, T. nativa, T. britovi and T. pseudospiralis. In order to identify a genetic variability in ecologically distinct species of Trichinella, which may lead to a potential geographical disposition of Trichinella isolates, we test the variability of nine nuclear microsatellite loci, in a small population of Trichinella isolates of Romania. As a result, we obtained a positive amplification for most of the microsatellites studied for Trichinella strains belonging to different species (T.spiralis, T.britovi). We noticed a size-difference between the amplicons generated by the DNA of various Trichinella species (T.spiralis vs T.britovi).We identify a genetic variability inside the same Trichinella population (T.spiralis), differentiating 3 strains (7.97, 7.42,7.29) by 2 microsatellites (TP43, TP32). For a more detailed analysis of the differences obtained in this study, we plan to use the fluorescently (6-FAM) marked primers, for sequencing and genotyping of the amplification products.