The aim of this paper was to reach the following objectives: macroscopic and microscopic assay of stallion semen destined for cryopreservation, assessment of viability and morphology of spermatozoa using two staining methods (intravital with eosin and Spermac® - MiniTubtm) and comparative evaluation of the results for the two staining methods. The samples were collected from ten clinically healthy stallions 5 to 10 years of age, of different breeds. Semen has been macroscopically evaluated, determining the volume, color, smell and viscosity. Microscopic evaluation consisted of determining motility, viability, density, concentration and total spermcells number, as well as normal or pathologic morphology description of spermatozoa (using the two staining methods). The following conclusions can be drawn from our research: the volume of collected semen varied between 40 and 150 ml, motility varied between 50 and 90%, only the samples above 70% being suitable for cryopreservation. There was no direct correlation between the volume of the ejaculate and the total number of spermatozoa from the ejaculate. Citomorphologically, the stallions considered for the study showed a high level of homogenity. Even though the eosin stain is preferable for differentiating living from dead spermatozoa, Spermac® stain allows a more accurate morphologic evaluation